ABSTRACT
The leaves of Andrographis paniculata acanthaceae is used locally in Northern Nigerian for the treatment of hypertension without quantification or dosage standardization. This work was primarily set out to find justification for the local medicinal use of this plant and to scientifically study the pharmacological activity of the plant and formulate it into a suitable tablet dosage form, as a means of standardization of the dosage regimen. Phytochemical, pharmacological and formulation studies of the leave, aqueous and Methanolic extracts of the plant leaves were carried out using standard methods. Phytochemical screening as described in official monograph was carried out to detect the presence of secondary metabolites. Extracts were tested for antihypertensive activity in cats by monitoring changes in blood pressure following administration of the extract. The antihypertensive activity was compared with propranolol (standard drug). Toxicity study was also carried out by determining the LD50 of the extract in rats. In formulation studies, micrometric properties of the extracts and granules such as size distribution, moisture content, angle of repose. Carr’s Index etc., were determined using different binders (maize starch, polyvinyl pyrollidone (PVP) and gelatin). Disintegrants (maize starch, pregelationised starch, microcrystalline cellulose, heavy magnesium carbonate and dicalcium phosphate) and diluents (Lactose and heavy magnesium carbonate) were also included in the formulations. Different methods of formulations were carried out, but only the best three of the Methanolic extracts were used for this work. Properties of the resulting tablets such as weight variation, crushing strength, disintegration properties, friability and dissolution profiles were determined and analyzed. Effects of storage condition (such as humidity and temperature) on the tablet properties were also determined. Stability studies on the extract were carried out using TLC technique and Differential Scanning Calorimetry (DSC). Results of the Phytochemical test showed that the plant extract contained alkaloids tanning, cardioglycosides, flavonoids and terpenes. The plant is relatively safe, with an LD50 of 1.90 x 104mg/kg body weight which is much higher than those of 0.81mg/kg body weight taken by the local people. Pharmacological studies showed that the leave extract possesses significant antihypertensive activity, comparable to Propranolol with methanol extract exhibiting greater activity. viii Qualitative and pharmaceutically acceptable tablets could only be produced from Methanolic extract using the wet granulation method. When excipients such as pregelatinzed starch (disintegrants), heavy magnesium carbonate (diluents) and 2.5% w/v gelatine binder was used. The thin layer chromatography and DSC studies showed that the crude extract contain three distinct components. The stability studies showed that the Methanolic extract tablets were stable under proper storage condition even after 4 years.
ABSTRACT
The management of current assets and short term funds is as important as that of fixed assets and long te...
Background of the Study
The success of corporations is influenced by a variety of factors, one of which...
ABSTARCT
Whatsapp is getting to be one of most tools for communication among people, where everyone can exchange, share, comment, discuss...
ABSTRACT
This study was conducted to examine the challenges and prospects of church planting in Nigeria. Church plan...
Abstract
Conventional wisdom holds that health is central to human general well-being, as well as a prerequisite for in...
BACKGROUND OF THE STUDY
Prior to 1993, only very little was known of Value Added Tax (VAT) in Nigeria....
ABSTRACT
The main aims and objectives of this study titled; “ ADMINISTRATION OF THE DEATH PENALTY IN THE CRIMINAL JUSTICE SYSTEM IN...
ABSTRACT
The essence of creation of man in any environment is to interact, relate and have dealings wit...
Abstract
Individuals all over the world continue to utilize traditional medical care, despite the increased availability of modern medici...
Background of study
As everybody has own aim to acquire income and show how he or she has income over t...